How to Win the Palo Alto Longevity Prize

$1,000,000 is the recently announced prize by Joon Yun, a Palo Alto-based entrepreneur, who is willing to donate this amount of money as an incentive to end aging. Half of the million will be given to the team of researchers who are able to extend lifespan by 50% in a model animal, and the other half – to those who manage to “demonstrate that it can restore homeostatic capacity (using heart rate variability as the surrogate measure) of an aging reference mammal to that of a young adult.”

Performing the experiment described below will secure winning the Homeostatic Capacity half of the Prize. The probability of the proposed study to demonstrate significant improvement of the heart rate variability marker is extremely high, because parabiosis was already shown to promote functional parameters of the nervous and cardiovascular systems. Now, by using a young clone we can reduce all possible immunological adverse reactions to the minimum and see how the old animal rejuvenates because of the circulating systemic factors produced by the young clone. Check out the detailed prize-winning study description here.

Heterochronic parabiosis for old mouse rejuvenation

One of the most productive paradigms of aging suppression is based on rejuvenation of blood-borne systemic regulatory factors. Parabiosis, which is characterized by a shared blood supply between two surgically connected animals, may provide such experimental paradigm. We propose to use heterochronic parabiosis, the parabiotic pairing of two animals of different ages, for old mouse rejuvenation. Heterochronic parabiosis also provides an experimental system to identify systemic factors influencing the aging process of the old mouse and promoting its longevity. The optimal rejuvenation effect of heterochronic parabiosis can be achieved by using cloned (genetically identical) animals. This will help avoid potential side effects caused by immune response.

Parabiosis experiment chart

The early reported studies that used heterochronic parabiosis in rodent models to study lifespan regulation provided evidence of significant benefit to the older parabiont (reviewed in Conboy et al., 2013; Eggel, Wyss-Coray, 2014). Heterochronic parabiosis resulted not only in lifespan extension of the older parabiont (Ludwig & Elashoff, 1972), but it also promoted functional and regeneration potential in the aging central nervous system (Ruckh et al., 2012; Villeda et al., 2011), muscle and liver (Conboy et al., 2005), reversed age-related cardiac hypertrophy (Loffredo et al., 2013) and some other age-related parameters. Thus heterochronic parabiosis experiments indicate that blood-borne signals from a young circulation can significantly impact the function of aging tissues. The implication of these findings is that old tissues might make their function almost as well as young tissues if, by means of systemic influences, the molecular pathways could be ‘rejuvenated’ from an old state to a young state.

The optimum rejuvenation effect of heterochronic parabiosis can be achieved using genetically identical animals. Genetically identical non-model organisms of different age can only be obtained by cloning. Interestingly, that there are no investigations of heterochronic parabiosis of cloned animals.

The aim of the project is the comprehensive investigation of rejuvenation potential of cloned mice heterochronic parabiosis.

Research plan:

At first we will perform cloning of adult (1-year-old) mice using technique for improved success cloning rate (Mizutani et al., 2014).

The study is performed in five groups of animals:

  1. Pair of cloned young and old heterochronic parabionts.
  2. Pair of young and old heterochronic parabionts (not cloned).
  3. Pair of two young parabionts.
  4. Pair of two old parabionts.
  5. Intact control animals.

The parabiosis is established at the age of 18 months for old partners and 2 month for the young ones. The detailed life span assay reveals the influence of heterochronic parabiosis with young clone on cardiovascular, nervous, respiratory, skeletal and muscular systems. The lifespan assay shows the young clone parabiosis impact on longevity of older partner.

In addition, systemic factors, which influence the aging process of the old mouse and promote its longevity and rejuvenation, are revealed.

Expected results:

  • Study of heterochronical parabiosis effects on the process of cell and tissue aging, development of age-related diseases, and other age-related parameters including organismal longevity of the old mouse
  • Identification of rejuvenation factors
  • Results of the experiment may be used for development of human rejuvenation approach by systemic regulation of the aging process


Filed under Biology of Aging

5 responses to “How to Win the Palo Alto Longevity Prize

  1. Thank you for the tips Maria Konovalenko Esquiress; That would be amazing. A 500,000.00 or million would set the stage for agings now end. At Adam and Eve Word Research… Yet, I have no human to show them that has been made rejuvenated by my elixir.. {My method amazingly is all organic, and could be tested on humans as trial. And it is guaranteed to work. This formula was found in past historical and in past Scientific research} I am what one must call on the ground floor of development, as a lab/institute.. {Hence todays garage sale..Ha} But with that much funding I could start a small group of people, unto the road to recovery/healing/rejuvenating with just that much funding.. Heading towards eons/centuries of Life… {I lost part of my website due to my equipment, so I had to nearly start from scratch go view … Respect}

    • Wait, it’s a trap! No seriously, do not click on the link if you don’t want to swim in knee deep BS. Maria I hope you block the scammers. Also – nice article as always, it’s a pleasure to read you blog, keep it up. 😉

      • Its so odd, how so many can speak of longevity, and life extension, and immortality.. Then when some one finds the cure, they say that’s impossible. And them not knowing that is the protrance of death/quorum sensing of the infection itself… Keeping them from Extended Life/Immortality… Respect

  2. Eugene

    For a real, valid US Clinical Trial of something similar to heterochronic parabiosis (more like blood-transfusion, but the same young-person to old-person blood-sharing principle applies), please see the following link

    (or more safely, go to, and search for “Alkahest”.

    The name of the study (currently recruiting volunteers with Mild-to-Moderate Alzheimer’s) is “The PLasma for Alzheimer SymptoM Amelioration (PLASMA) Study”.

    This is real progress. Let’s hope for the best.

    Maria, thank you and stay well,

  3. Kurt

    How much of the benefit is coming from the doubled immunological space and the increased production of naive T-cells from the young mouse?

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