Do Model Animals Tell Us Anything about Human Aging?


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I wrote a tiny report on the pluses and minuses of using model  animals in aging research.

Using model animals in gerontological studies has yielded an enormous wealth of useful information about the mechanisms of human aging and longevity. Animal models were crucial in identifying the conserved pathways that regulate human aging. Model organisms are fundamental for aging research, because there are serious limitations of using human subjects, such as the length of lifespan, genetic heterogeneity and vast differences in environmental influences. The shape of survival curves represents the health of the organism over time. Model organisms display significantly different lifespans, however the survival curves resemble those of humans quite remarkably. Despite this general similarity in the way we describe aging between humans and model animals, there are some distinct differences (Mitchell, Scheibye-Knudsen, Longo, & de Cabo, 2015). For instance, increasing Sir2 gene expression in yeast (Kaeberlein, McVey, & Guarente, 1999), nematodes (Tissenbaum & Guarente, 2001), and flies (Rogina & Helfand, 2004) boosts animal longevity. A small molecule called resveratrol was found to activate Sir2 and its mammalian ortholog SIRT1 (Howitz et al., 2003). Resveratrol extends lifespan of mice fed a high-fat diet (Baur et al., 2006), however it failed to have a beneficial longevity effect in mice on a standard diet (Pearson et al., 2008). This example highlights the fact that we cannot simply transfer the results of longevity interventions to humans and expect the same efficacy as in invertebrate models.

Everyday the researchers are broadening the understanding of human biology of aging with the help of various model systems. Each of them has its advantages and drawbacks. Let’s take a look at what those are for the most widely used animal models.

Yeast S.cerevisiae

Yeast have been instrumental in identifying the major conserved aging pathways shared among a large variety of species (Wasko & Kaeberlein, 2014). Despite the fact that yeast is a unicellular organism that has significant differences in its genetic pathways with humans, the advantages of using yeast as an aging model include their fast growth, low cost and easy storage and maintenances of organism strains. Over the years researchers have developed a broad variety of genetic manipulations that make yeast a powerful tool in the hands of an aging biologist.

Yeast replicative lifespan essay is based on the asymmetric division of mother and daughter yeast cells and was used to identify various types of factors contributing to senescence. The initial findings named the accumulation of extra-ribosomal DNA circles as the main type of damage that is inherited by the mother cell in the course of the asymmetric division. Later on a few additional types of accumulated damage were demonstrated to contribute to aging of the mother cells, such as damaged nuclear pore complexes (Kaeberlein, 2008; Shcheprova, Baldi, Frei, Gonnet, & Barral, 2008), cytosolic aggregates and oxidized proteins (Aguilaniu, Gustafsson, Rigoulet, & Nystrom, 2003; Erjavec & Nystrom, 2007) and dysfunctional mitochondria (Delaney et al., 2013; Lai, Jaruga, Borghouts, & Jazwinski, 2002). Establishing the exact role of these molecular damages in human aging remains an open question.

Nematode C.elegans

The roundworm Caenorhabditis elegans are a powerful model for studying aging due to their short lifespan, which is approximately 17 days at 20 °C (Tissenbaum, 2015). They are easy to culture and maintain strains, because nematodes can be kept frozen and suffer no apparent damage upon thawing. The animals are optically transparent can be used in high-throughput experiments, which makes them a perfect tool for answering the most pressing questions in biology of aging (Spivey & Finkelstein, 2014). Two RNAi libraries that cumulatively cover about 80% of C.elegans genome (Kamath et al., 2003; Rual et al., 2004) provide an excellent tool for identification of evolutionary conserved mechanisms of aging.

However, there are obvious drawbacks of using C. elegans as a model for human aging. They are evolutionary distant from humans, lack tissues like brain, blood, they don’t have internal organs and are post-mitotic, meaning that nematodes lack the ability to regenerate their tissues and are limited in serving as a model of aging of highly proliferative tissues.

Nonetheless, C.elegans will remain a widely used system for elucidating aging mechanisms in part due to the possibility of performing whole genome screenings and the fact that these animals have been instrumental in identifying genetic pathways governing aging. In fact, at least three of the major aging pathways, including insulin/IGF-1 signaling (Kenyon, Chang, Gensch, Rudner, & Tabtiang, 1993; Morris, Tissenbaum, & Ruvkun, 1996), TOR pathway (Vellai et al., 2003), and SIR2 pathway (Tissenbaum & Guarente, 2001) were identified in C.elegans. Further studies of C.elegans biology of aging will increase our understanding of the common pathways between the model organisms and humans (Kennedy & Pennypacker, 2014)

Fruit fly D.melanogaster

Fruit flies have many advantages as a model system for aging studies. They have a relatively short lifespan of 60-80 days, which is more than that of a nematode, but compared to them drosophila have more distinct tissues and organs including the brain, eyes, kidney, liver and heart. Fruit flies have proliferating stem cell populations in their guts. Females can produce up to 200 eggs in the first 10 days, and they acquire reproductive ability within the first 10 days, which speaks of their high fecundity. Flies share about 60% of disease-related genes with humans (Matthews, Kaufman, & Gelbart, 2005), which makes them a desirable model also given their low cost and easy handling. There are several genetic systems that are used to create transgenic lines and manipulate single gene expression like UAS-GAL4 system, RNAi and dominant enchanser screening (Duffy, 2002; St Johnston, 2002). However, maintaining a transgenic strain is more costly and labor-heavy, since whole flies cannon be frozen and thawed without damage.

Hydra

Hydra is definitely not the most popular model organism, but it might be overlooked quite groundlessly. Hydras are notorious for their negligible senescence. This very fact makes them a very desirable system to study. In fact, there is no apparent senescence in asexually reproducing hydras, yet the signs of aging can be seen after the organism reproduces sexually, such as loss of neurons and feeding behavior, interstitial somatic stem cell number reduction as well as disorganization of actin myofibers and loss of contractility (Yoshida, Fujisawa, Hwang, Ikeo, & Gojobori, 2006). Another overlooked fact is that hydras share 6071 genes with humans, whereas fruit flies have 5696 genes in common with humans, and nematodes – only 4751 (Wenger & Galliot, 2013). Among the known human aging genes at least 80% are shared with hydra (Tomczyk at al, 2014). Hydras may shed light on the mechanisms of exceptional longevity and the processes that regulate switching from a ‘non-aging’ to senescent phenotypes, which may eventually inform our understanding of molecular mechanisms of aging in humans.

Fish

The most widely used fish model is the zebrafish D.rerio. It lives for about 2-3 years, which is not particularly beneficial, because its lifespan is similar of rodents, but it is more evolutionary distant from humans. Nonetheless, zebrafish has a remarkable ability to regenerate its tissues, which is an advantage for elucidating the mechanisms of tissue regeneration and longevity (Lepilina et al., 2006).

Another fish may be a more promising laboratory model for aging – turquoise killifish Nothobranchius furzeri. Killifish is one of the shortest-lived vertebrate with a lifespan of only 13 weeks (Gerhard, 2007). Its small size and high fecundity offer a considerable advantage in terms of reducing laboratory costs on housing and maintenance. A large number of age-related changes have been described in N.furzeri, and interestingly, the fish develops a high incidence of tumors with aging (Lucas-Sanchez, Almaida-Pagan, Mendiola, & de Costa, 2014). Turquoise killifish is responsive to calorie restriction and has increased longevity after administrating resveratrol with the standard diet (Terzibasi, Valenzano, & Cellerino, 2007).

Rodents

Mice are invaluable in aging research. There are approximately 99% of human orthologs in mice, which is a significant advantage compared to invertebrate models. Mouse lifespan is approximately 2-3 years depending on the strain, which makes them a more expensive tool in the arsenal of an aging biologist. Inbred mice have been studies very extensively and a large body of knowledge about aging mechanisms, age-related diseases and existing and potential therapies was created using this model. Using inbred lines is a double-edged sword: on one hand, genetic differences between animals are virtually non-existent, however this is not representative of human population and it is not clear to what extent the results can be transferred to humans. For example, about 70% of published experiments were done in

C57BL/6 mouse strain, that has its peculiarities being an inbred strain, like high prevalence of lymphomas and increased susceptibility to metabolic dysregulation (Ward, 2006).

Rats have been studied in research setting in many areas, including age-related pathologies like cardiovascular diseases, cancer, neurological and renal disorders, as well as for behavioral studies of cognition. Both inbred and outbred lines are used in research. Interestingly, not all longevity interventions are successful in rats, for example, metformin extends lifespan in mice, but not in F344 rats (Martin-Montalvo et al., 2013; Smith et al., 2010).

Naked mole rats

Heterocephalus glaber, the naked mole rat, is the most long-lived rodent with a maximum life span of approximately 30 years(Jarvis, 1981). Naked mole rat exhibits negligible senescence, virtually no age-related increase in mortality and high reproduction levels until death (Mitchell et al., 2015). They have several signs of age-related pathology similar to humans, such as osteoarthritis and degeneration of the retina (Edrey, Hanes, Pinto, Mele, & Buffenstein, 2011). Naked mole rats can provide clues to mechanisms of longevity and potential therapies in humans, and hence are an extremely valuable model animal. There are several disadvantages of using them as laboratory animals, including specific housing conditions like low light levels, high temperature and humidity. Naked mole rats are eusocial animals; therefore they should be housed in colonies resembling undergrounds tunnels that they create in their natural habitat. Very long lifespan poses an obvious limitation on the variety of experiments suitable for this model, however conditions for naked mole rat cell culturing are described and genome of this animal is sequenced, which makes it a desirable system to study to elucidate the mechanisms of escaping age-related disorders.

Primates

Non-human primates are the closest animals to humans in terms of genetic, metabolic and behavioral differences. Rhesus macques have been used in various types of research, however there are not too many studies of age-related mechanisms in primates. The main reasons for that are their long lifespan, which is more than 30 years, their size and weight, which complicate housing and maintenance and make this model an expensive and hard to handle (Mitchell et al., 2015). However, there are several distinct advantages of using non-human primates for studying age-related pathologies, such as Alzheimer’s disease and other neurodegenerative diseases that can’t be recapitulated in mouse models. Primates have Aβ and tau aggregates and cortical atrophy (Heuer, Rosen, Cintron, & Walker, 2012), whereas wild type mice don’t show protein aggregation, which makes non-human primates a valuable model for studying aging of the brain (Verdier et al., 2015).

References:

Aguilaniu, H., Gustafsson, L., Rigoulet, M., & Nystrom, T. (2003). Asymmetric inheritance of oxidatively damaged proteins during cytokinesis. Science, 299(5613), 1751-1753. doi: 10.1126/science.1080418

Baur, J. A., Pearson, K. J., Price, N. L., Jamieson, H. A., Lerin, C., Kalra, A., . . . Sinclair, D. A. (2006). Resveratrol improves health and survival of mice on a high-calorie diet. Nature, 444(7117), 337-342. doi: 10.1038/nature05354

Delaney, J. R., Murakami, C., Chou, A., Carr, D., Schleit, J., Sutphin, G. L., . . . Kaeberlein, M. (2013). Dietary restriction and mitochondrial function link replicative and chronological aging in Saccharomyces cerevisiae. Exp Gerontol, 48(10), 1006-1013. doi: 10.1016/j.exger.2012.12.001

Duffy, J. B. (2002). GAL4 system in Drosophila: a fly geneticist’s Swiss army knife. Genesis, 34(1-2), 1-15. doi: 10.1002/gene.10150

Edrey, Y. H., Hanes, M., Pinto, M., Mele, J., & Buffenstein, R. (2011). Successful aging and sustained good health in the naked mole rat: a long-lived mammalian model for biogerontology and biomedical research. ILAR J, 52(1), 41-53.

Erjavec, N., & Nystrom, T. (2007). Sir2p-dependent protein segregation gives rise to a superior reactive oxygen species management in the progeny of Saccharomyces cerevisiae. Proc Natl Acad Sci U S A, 104(26), 10877-10881. doi: 10.1073/pnas.0701634104

Gerhard, G. S. (2007). Small laboratory fish as models for aging research. Ageing Res Rev, 6(1), 64-72. doi: 10.1016/j.arr.2007.02.007

Heuer, E., Rosen, R. F., Cintron, A., & Walker, L. C. (2012). Nonhuman primate models of Alzheimer-like cerebral proteopathy. Curr Pharm Des, 18(8), 1159-1169.

Howitz, K. T., Bitterman, K. J., Cohen, H. Y., Lamming, D. W., Lavu, S., Wood, J. G., . . . Sinclair, D. A. (2003). Small molecule activators of sirtuins extend Saccharomyces cerevisiae lifespan. Nature, 425(6954), 191-196. doi: 10.1038/nature01960

Jarvis, J. U. (1981). Eusociality in a mammal: cooperative breeding in naked mole-rat colonies. Science, 212(4494), 571-573.

Kaeberlein, M. (2008). Cell biology: A molecular age barrier. Nature, 454(7205), 709-710. doi: 10.1038/454709a

Kaeberlein, M., McVey, M., & Guarente, L. (1999). The SIR2/3/4 complex and SIR2 alone promote longevity in Saccharomyces cerevisiae by two different mechanisms. Genes Dev, 13(19), 2570-2580.

Kamath, R. S., Fraser, A. G., Dong, Y., Poulin, G., Durbin, R., Gotta, M., . . . Ahringer, J. (2003). Systematic functional analysis of the Caenorhabditis elegans genome using RNAi. Nature, 421(6920), 231-237. doi: 10.1038/nature01278

Kennedy, B. K., & Pennypacker, J. K. (2014). Drugs that modulate aging: the promising yet difficult path ahead. Transl Res, 163(5), 456-465. doi: 10.1016/j.trsl.2013.11.007

Kenyon, C., Chang, J., Gensch, E., Rudner, A., & Tabtiang, R. (1993). A C. elegans mutant that lives twice as long as wild type. Nature, 366(6454), 461-464. doi: 10.1038/366461a0

Lai, C. Y., Jaruga, E., Borghouts, C., & Jazwinski, S. M. (2002). A mutation in the ATP2 gene abrogates the age asymmetry between mother and daughter cells of the yeast Saccharomyces cerevisiae. Genetics, 162(1), 73-87.

Lepilina, A., Coon, A. N., Kikuchi, K., Holdway, J. E., Roberts, R. W., Burns, C. G., & Poss, K. D. (2006). A dynamic epicardial injury response supports progenitor cell activity during zebrafish heart regeneration. Cell, 127(3), 607-619. doi: 10.1016/j.cell.2006.08.052

Lucas-Sanchez, A., Almaida-Pagan, P. F., Mendiola, P., & de Costa, J. (2014). Nothobranchius as a model for aging studies. A review. Aging Dis, 5(4), 281-291. doi: 10.14336/AD.2014.0500281

Martin-Montalvo, A., Mercken, E. M., Mitchell, S. J., Palacios, H. H., Mote, P. L., Scheibye-Knudsen, M., . . . de Cabo, R. (2013). Metformin improves healthspan and lifespan in mice. Nat Commun, 4, 2192. doi: 10.1038/ncomms3192

Matthews, K. A., Kaufman, T. C., & Gelbart, W. M. (2005). Research resources for Drosophila: the expanding universe. Nat Rev Genet, 6(3), 179-193. doi: 10.1038/nrg1554

Mitchell, S. J., Scheibye-Knudsen, M., Longo, D. L., & de Cabo, R. (2015). Animal models of aging research: implications for human aging and age-related diseases. Annu Rev Anim Biosci, 3, 283-303. doi: 10.1146/annurev-animal-022114-110829

Morris, J. Z., Tissenbaum, H. A., & Ruvkun, G. (1996). A phosphatidylinositol-3-OH kinase family member regulating longevity and diapause in Caenorhabditis elegans. Nature, 382(6591), 536-539. doi: 10.1038/382536a0

Pearson, K. J., Baur, J. A., Lewis, K. N., Peshkin, L., Price, N. L., Labinskyy, N., . . . de Cabo, R. (2008). Resveratrol delays age-related deterioration and mimics transcriptional aspects of dietary restriction without extending life span. Cell Metab, 8(2), 157-168. doi: 10.1016/j.cmet.2008.06.011

Rogina, B., & Helfand, S. L. (2004). Sir2 mediates longevity in the fly through a pathway related to calorie restriction. Proc Natl Acad Sci U S A, 101(45), 15998-16003. doi: 10.1073/pnas.0404184101

Rual, J. F., Ceron, J., Koreth, J., Hao, T., Nicot, A. S., Hirozane-Kishikawa, T., . . . Vidal, M. (2004). Toward improving Caenorhabditis elegans phenome mapping with an ORFeome-based RNAi library. Genome Res, 14(10B), 2162-2168. doi: 10.1101/gr.2505604

Shcheprova, Z., Baldi, S., Frei, S. B., Gonnet, G., & Barral, Y. (2008). A mechanism for asymmetric segregation of age during yeast budding. Nature, 454(7205), 728-734. doi: 10.1038/nature07212

Smith, D. L., Jr., Elam, C. F., Jr., Mattison, J. A., Lane, M. A., Roth, G. S., Ingram, D. K., & Allison, D. B. (2010). Metformin supplementation and life span in Fischer-344 rats. J Gerontol A Biol Sci Med Sci, 65(5), 468-474. doi: 10.1093/gerona/glq033

Spivey, E. C., & Finkelstein, I. J. (2014). From cradle to grave: high-throughput studies of aging in model organisms. Mol Biosyst, 10(7), 1658-1667. doi: 10.1039/c3mb70604d

St Johnston, D. (2002). The art and design of genetic screens: Drosophila melanogaster. Nat Rev Genet, 3(3), 176-188. doi: 10.1038/nrg751

Terzibasi, E., Valenzano, D. R., & Cellerino, A. (2007). The short-lived fish Nothobranchius furzeri as a new model system for aging studies. Exp Gerontol, 42(1-2), 81-89. doi: 10.1016/j.exger.2006.06.039

Tissenbaum, H. A., & Guarente, L. (2001). Increased dosage of a sir-2 gene extends lifespan in Caenorhabditis elegans. Nature, 410(6825), 227-230. doi: 10.1038/35065638

Tomczyk S., Fischer K., Austad S., Galliot B. (2015) Hydra, a powerful model for aging studies, Invertebrate Reproduction & Development, 59:sup1, 11-16, DOI: 10.1080/07924259.2014.927805

Vellai, T., Takacs-Vellai, K., Zhang, Y., Kovacs, A. L., Orosz, L., & Muller, F. (2003). Genetics: influence of TOR kinase on lifespan in C. elegans. Nature, 426(6967), 620. doi: 10.1038/426620a

Verdier, J. M., Acquatella, I., Lautier, C., Devau, G., Trouche, S., Lasbleiz, C., & Mestre-Frances, N. (2015). Lessons from the analysis of nonhuman primates for understanding human aging and neurodegenerative diseases. Front Neurosci, 9, 64. doi: 10.3389/fnins.2015.00064

Ward, J. M. (2006). Lymphomas and leukemias in mice. Exp Toxicol Pathol, 57(5-6), 377-381. doi: 10.1016/j.etp.2006.01.007

Wasko, B. M., & Kaeberlein, M. (2014). Yeast replicative aging: a paradigm for defining conserved longevity interventions. FEMS Yeast Res, 14(1), 148-159. doi: 10.1111/1567-1364.12104

Wenger, Y., & Galliot, B. (2013). RNAseq versus genome-predicted transcriptomes: a large population of novel transcripts identified in an Illumina-454 Hydra transcriptome. BMC Genomics, 14, 204. doi: 10.1186/1471-2164-14-204

Yoshida, K., Fujisawa, T., Hwang, J. S., Ikeo, K., & Gojobori, T. (2006). Degeneration after sexual differentiation in hydra and its relevance to the evolution of aging. Gene, 385, 64-70. doi: 10.1016/j.gene.2006.06.031

10 Comments

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10 responses to “Do Model Animals Tell Us Anything about Human Aging?

  1. WOW Thank you so much Maria!!! I, as most know of all the research model animals, but you gathering Data and putting them in line and perspective.. Helps me tremendously… {Me being a Data Researcher/Solutions analyst. I love Compiled Data from a reliable Source}

    We have learned much about mans diseases through model specimens. But we found the causes of ageing and death long ago by studying mankind/WOMANKIND… And had the causes theorized to us as far back as the Days of Hippocrates…

    As I search still for financing {and am hopefully closer to that} I will be contacting you.. To see you and My Wife even more vibrant and Physically distinctive will be an amazing thing.. I will be placing/sharing this link/paper/treatise on my New group site… At https://www.facebook.com/groups/899582440105597/ … Respect to the Wonder Woman of Transhumanism/Life Extension Maria Konovalenko

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  3. Good post – I really liked it!

    One question though: “whereas wild type mice don’t show protein aggregation, which makes non-human primates a valuable model for studying aging of the brain” => don’t mice also suffer protein aggregation? See http://www.ncbi.nlm.nih.gov/pubmed/21321389. It’s just that they don’t seem to show as much phenotypic consequence from said aggregation.

  4. Great topic! Thank you for putting this valuable information together here. It will serve very well for future reference.

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  8. john p cooke

    Wonderful overview of animal models, thanks Maria!
    John Cooke
    Director, Center for Cardiovascular Regeneration
    Houston Methodist Research Institute

  9. Aric

    As an outsider I found this quite interesting. David Brin (a layman in this field) believes that research on most short-lived animals will not help for finding easy ways to increase human longevity because most of the ‘switches’ which increase lifespans in other animals have already been turned on in humans. I wonder what your response would be to that.

  10. this is really nice article

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